Protein_Domain

Part:BBa_J119001:Design

Designed by: Caleb J. Carr   Group: Eckdahl Lab   (2011-07-21)


BsmBI-RBS-GFP1-BsaI


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1
    Illegal PstI site found at 402
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal PstI site found at 402
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1
    Illegal PstI site found at 402
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1
    Illegal PstI site found at 402
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 396


Design Notes

This is not a BioBrick part. It was designed and produced for the specific purpose of solving multi-node Hamiltonian Path Problems with the computing power of E Coli.


Source

PCR Amplification of the GFP template split with specific oligos to result in the full noted 407 nt sequence.

References